Activation of nuclear factor kappaB signaling pathway in human osteoblasts responses to Staphylococcus aureus in vitro / 中华外科杂志
Chinese Journal of Surgery
;
(12): 264-267, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-257512
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether S. aureus could activate NF-κB signaling pathway in human osteoblasts.</p><p><b>METHODS</b>Immunoblot and electrophoretic mobility shift assay were used to detect the degradation of I-κBα and activation of NF-κB in human osteoblasts following infection with S.aureus, respectively, and there were investigated the activated state of NF-κB signaling pathway in human osteoblasts. In addition, enzyme-linked immunosorbent assay was used to measure the secretion of IL-6 in culture supernatants, which was represented as one of important cytokines in osteomyelitis, and an inhibitor of NF-κB, SN50, which was added to human osteoblasts culture prior to 1 hour at 50 µmol/L before the infection of S.aureus, was used to determine whether S.aureus-activated NF-κB signaling pathway regulates IL-6 secretion of human osteoblasts.</p><p><b>RESULTS</b>S.aureus could induce the degradation of I-κBα (I-κBα(15 min)/I-κBα(0 min) = 0.409 ± 0.245 and I-κBα(30 min)/I-κBα(0 min) = 0.061 ± 0.010) and activation of NF-κB in human osteoblasts in a time and dose-dependent manner following infection. In addition, the secretion of IL-6 in the supernatants of human osteoblasts ((2.17 ± 0.11) µg/L) was suppressed by 50 µmol/L SN50 compared to without the addition of SN50 ((3.58 ± 0.31) µg/L) (F = 174.25, P < 0.05).</p><p><b>CONCLUSIONS</b>S.aureus could activate NF-κB signaling pathway in human osteoblasts, which could regulate cytokines secretions of human osteoblasts.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Staphylococcal Infections
/
Signal Transduction
/
Cells, Cultured
/
NF-kappa B
/
Interleukin-6
/
Bodily Secretions
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2012
Type:
Article
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