Expression of vascular endothelial cell growth factor and its receptor mRNA in breast cancer tissues / 中华外科杂志
Chinese Journal of Surgery
;
(12): 119-121, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-257714
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of vascular endothelial cell growth factor (VEGF) and its receptor FLT-1, FLK-1 mRNA in breast cancer tissues and their correlation with clinicopathological factors.</p><p><b>METHODS</b>The expression of VEGF and and its receptor FLT-1, FLK-1 mRNA were analyzed by reverse transcription - polymerase chain reaction (RT-PCR) in the specimens from 47 patients with breast cancer and 11 patients with benign breast disease.</p><p><b>RESULTS</b>VEGF121, 165 mRNA were all detected in malignant and benign breast tissues, with higher level, (0.420 +/- 0.133 and 0.291 +/- 0.094 respectively) in breast cancer tissues than in benign breast tissues, [0.196 +/- 0.067 (P = 0.000) and 0.206 +/- 0.058 (P = 0.001) respectively]. VEGF121 mRNA expression was stronger than VEGF165 mRNA expression (P = 0.000) in breast cancer tissues, whereas there is no significant difference in benign breast tissues (P = 0.666). FLT-1, FLK-1 mRNA were expressed in 18 of 47 (38.3%) and 12 of 47 (25.5%) breast cancer tissues respectively, but not in benign breast tissues. Moreover, no correlation was observed between the expression of VEGF121, VEGF165, FLT-1, FLK-1 mRNA in breast cancer tissues and patients' age, tumor size, lymph node metastasis, tumor stages, estrogen or progesterone receptor status.</p><p><b>CONCLUSION</b>The expression of VEGF and its receptor FLT-1, FLK-1 mRNA were up-regulated in breast cancer tissues, suggesting its important role in angiogenesis of breast cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Breast Neoplasms
/
RNA, Messenger
/
Receptors, Progesterone
/
Receptors, Estrogen
/
Reverse Transcriptase Polymerase Chain Reaction
/
Vascular Endothelial Growth Factor Receptor-1
/
Vascular Endothelial Growth Factor A
/
Genetics
/
Metabolism
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS