Correlation between MMP-2 activation and MT1-MMP mRNA expression in thymic epithelial tumors / 中华病理学杂志
Chinese Journal of Pathology
; (12): 18-23, 2006.
Article
in Zh
| WPRIM
| ID: wpr-258237
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between activation of pro-MMP-2 and expression of matrix metalloproteinases (MMP)-2, MT1-MMP and tissue inhibitor of metalloproteinases (TIMP)-2 mRNA in thymoma and thymic carcinoma; and to study the molecular mechanism of invasion and metastasis of thymic epithelial tumors.</p><p><b>METHODS</b>Fresh tissue specimens of thymoma, thymic carcinoma and normal thymus were included. The mRNA expression of MMP-2, MT1-MMP and TIMP-2 were analyzed by real-time reverse transcription polymerase chain reaction. The pro-MMP-2 activation ratio and its localization were determined by gelatin zymography and film in-situ gelatin-Zymography, respectively. Correlation of mRNA expression of MMP-2, MT1-MMP and TIMP-2 was investigated in tumors with different histological subtypes and clinical stages.</p><p><b>RESULTS</b>There were no significant differences in the expressions of MMP-2, MT1-MMP and TIMP-2 mRNA between I and II stage or III and IV stage thymomas (P > 0.05). However, significant differences of the expressions were observed between three tumor groups: I-II stage, III-IV stage and thymic carcinomas (P < 0.005), and between three histological subtypes: AB-B1 (lymphocyte-rich and mixed types), B2-B3 (cortical and predominantly polygonal cells types) and thymic carcinomas (P < 0.05). Expression levels of MT1-MMP and TIMP-2 mRNA were correlated with pro-MMP-2 activation ratio (Spearman rank correlation: r = 0.7235, r = 0.7647, P < 0.005). The expression of MMP-9 did not show significant differences between thymomas and thymic carcinomas.</p><p><b>CONCLUSIONS</b>MMP-2, MT1-MMP and TIMP-2 mRNA expression levels are correlated with the histologic subtypes and clinical stages of thymoma. The mRNA expressions of MT1-MMP and TIMP-2 are correlated with the activation ratio of pro-MMP-2. It is speculated that upregulation of MT1-MMP gene expression may induce an activation of pro-MMP-2 through TIMP-2.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Thymoma
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Thymus Gland
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Thymus Neoplasms
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RNA, Messenger
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Carcinoma, Squamous Cell
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Classification
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Tissue Inhibitor of Metalloproteinase-2
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Matrix Metalloproteinase 2
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Matrix Metalloproteinases
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2006
Type:
Article