Human neuroblastoma cells transfected with two Chinese presenilin 1 mutations are sensitized to trophic factor withdrawal and protected by insulin-like growth factor-1 / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 910-915, 2008.
Article
in English
| WPRIM
| ID: wpr-258567
ABSTRACT
<p><b>BACKGROUND</b>Two novel presenilin 1 (PS1) mutations, V97L and A136G, were recently found to be involved in the early-onset of Alzheimer's disease in two Chinese families. This research aimed to verify their pathological effects.</p><p><b>METHODS</b>The human neuroblastoma SH-SY5Y cells stably transfected with these two Chinese presenilin 1 mutations were established to explore whether they are sensitive to, or influenced by, serum deprivation and protected by insulin-like growth factor-1 (IGF-1). Apoptosis rate, glucose uptake of the cells and the expression of glucose transport protein 1 (GLUT1) on cell membranes were examined.</p><p><b>RESULTS</b>The V97L or A136G mutants significantly decreased the cells viability and increased the apoptosis rate when compare to PS1wt and mock transfected cells. IGF-1 was found to improve the viability of these two kinds of mutant cells significantly, and to show a protective effect for the mutants when they were treated with trophic deprivation. The glucose uptake of each transfected cell line increased to about 25% after IGF-1 treatment, GLUT1 expression on the cell membrane increased modestly by about 15% - 20%.</p><p><b>CONCLUSIONS</b>Enhanced sensitivity to trophic withdrawal in the cells transfected with the two Chinese PS1 mutations may contribute to the neuron apoptosis. IGF-1 provided a protective effect to cells, possibly through an enhanced glucose transport and mitochondrial activities.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Insulin-Like Growth Factor I
/
Pharmacokinetics
/
Cell Survival
/
China
/
Blotting, Western
/
Apoptosis
/
Cell Line, Tumor
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Chinese Medical Journal
Year:
2008
Type:
Article
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