Relationship between hydrogen sulfide and myocardial damage in endotoxemic rats / 生理学报
Acta Physiologica Sinica
;
(6): 357-362, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-258644
ABSTRACT
To investigate the changes and role of hydrogen sulfide (H2S) in myocardial damage in endotoxemic rats, a rat model of endotoxemia induced by injection of lipopolysaccharide (LPS) was developed. Male Wistar rats were divided into four groups control group, LPS group, LPS + propargylglycine (PPG, a metabolic enzyme inhibitor of H2S) group and LPS + NaHS (H2S donor) group. The mean arterial pressure (MAP) of rats within 4 h was observed, TNF-alpha and H2S contents in plasma, TNF-alpha and H2S contents, lactate dehydrogenase (LDH) and myeloperoxidase (MPO) activity in cardiac muscles were determined. The morphological structure of cardiac muscle was observed. Administration of LPS caused a sustained fall in MAP within 4 h, and significant increases in TNF-alpha and H2S contents in plasma (P<0.05). Plasmic H2S content was negatively correlated with MAP (r = -0.936, -0.913 and -0.908 at 1, 2 and 4 h, respectively, P<0.05). LPS also induced increases in TNF-alpha and H2S contents, LDH and MPO activity in cardiac muscles and myocardial damage. Treatment with PPG reduced the increases in TNF-alpha and H2S contents in plasma, TNF-alpha and H2S contents, LDH and MPO activity in cardiac muscles, ameliorated the hypotensive effect and myocardial damage caused by LPS administration (P<0.05). However, treatment with NaHS increased TNF-alpha and H2S contents in plasma, TNF-alpha and H2S contents, LDH and MPO activity in cardiac muscles, and aggravated the hypotensive action and tissue injuries caused by LPS administration (P<0.05). It is suggested that hypotension and myocardial damage in endotoxemic rats are partly induced by increase in H2S content.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Blood
/
Blood Pressure
/
Chemistry
/
Lipopolysaccharides
/
Tumor Necrosis Factor-alpha
/
Rats, Wistar
/
Peroxidase
/
Endotoxemia
/
Toxicity
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2007
Type:
Article
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