Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 374-378, 2016.
Article
in English
| WPRIM
| ID: wpr-258810
ABSTRACT
Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. The average recovery range was 88.5%-105.4% for spiked samples (10, 50, and 100 μg/kg), and the coefficient of variation was in the range of 7.5%-14.5%. The limit of detection of CLEIA was 9.4 μg/kg, and this method was compared with the liquid chromatography-tandem mass spectrometry method using naturally contaminated samples, producing a correlation coefficient of >0.95. We demonstrate a reliable CLIEA for the rapid screening of neomycin in milk.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drug Residues
/
Neomycin
/
Food Contamination
/
Chemistry
/
Immunoenzyme Techniques
/
Milk
/
Limit of Detection
/
Luminescent Measurements
/
Metabolism
/
Anti-Bacterial Agents
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2016
Type:
Article
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