Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 315-322, 2016.
Article
in English
| WPRIM
| ID: wpr-258817
ABSTRACT
<p><b>OBJECTIVE</b>Chlamydia psittaci is an avian respiratory pathogen and zoonotic agent. The wide prevalence of C. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies against C. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen.</p><p><b>METHODS</b>The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens.</p><p><b>RESULTS</b>The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively.</p><p><b>CONCLUSION</b>These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance of C. psittaci infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Psittacosis
/
Poultry Diseases
/
Bacterial Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Chickens
/
Chlamydophila psittaci
/
Sensitivity and Specificity
/
Diagnosis
/
Allergy and Immunology
/
Genetics
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS