Enhancement of Virus Replication in An Influenza A Virus NS1-Expresssing 293 Cell Line / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 224-228, 2016.
Article
in English
| WPRIM
| ID: wpr-258830
ABSTRACT
The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Influenza A virus
/
Physiology
/
Virus Cultivation
/
Virus Replication
/
Cell Line
/
Gene Expression Regulation
/
Viral Nonstructural Proteins
/
HEK293 Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2016
Type:
Article
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