Evaluation of Protective Effects of Bioactive Phytochemicals Against Methotrexate in Salmonella typhimurium TA1535/pSK1002 Coupled with Micronucleus Assay / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 148-152, 2016.
Article
in English
| WPRIM
| ID: wpr-258841
ABSTRACT
We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate (P<0.01). These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Salmonella typhimurium
/
Plant Extracts
/
Micronucleus Tests
/
Random Allocation
/
Methotrexate
/
Cytoprotection
/
Drug Evaluation, Preclinical
/
Phytotherapy
/
Antimetabolites, Antineoplastic
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2016
Type:
Article
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