Flexibility Analysis of Bacillus thuringiensis Cry1Aa / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 634-641, 2015.
Article
in English
| WPRIM
| ID: wpr-258897
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the flexibility and mobility of the Bacillus thuringiensis toxin Cry1Aa.</p><p><b>METHODS</b>The graph theory-based program Constraint Network Analysis and normal mode-based program NMsim were used to analyze the global and local flexibility indices as well as the fluctuation of individual residues in detail.</p><p><b>RESULTS</b>The decrease in Cry1Aa network rigidity with the increase of temperature was evident. Two phase transition points in which the Cry1Aa structure lost rigidity during the thermal simulation were identified. Two rigid clusters were found in domains I and II. Weak spots were found in C-terminal domain III. Several flexible regions were found in all three domains; the largest residue fluctuation was present in the apical loop2 of domain II.</p><p><b>CONCLUSION</b>Although several flexible regions could be found in all the three domains, the most flexible regions were in the apical loops of domain II.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Conformation
/
Temperature
/
Bacillus thuringiensis
/
Bacterial Proteins
/
Computer Simulation
/
Software
/
Cluster Analysis
/
Chemistry
/
Entropy
/
Endotoxins
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2015
Type:
Article
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