The Effects of Ethyl Pyruvate on Lipopolysaccharide-induced Acute Lung Injury / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 374-383, 2006.
Article
in Korean
| WPRIM
| ID: wpr-25901
ABSTRACT
BACKGROUND:
Ethyl pyruvate (EP) is a derivative of pyruvate that has recently been identified by both various in vitro and in vivo studies to have antioxidant and anti-inflammatory effects. The aim of this study was to determine the effect of EP on lipopolysaccharide (LPS)-induced acute lung injury (ALI).METHODS:
5 weeks old, male BALB/c mice were used. ALI was induced by an intratracheal instillation of LPS 0.5mg/Kg/50microliter of saline. The mice were divided into the control, LPS, EP+LPS, and LPS+EP groups. In the control group, balanced salt solution was injected intraperitoneally 30 minutes before or 9 hours after the intratracheal instillation of saline. In the LPS group, a balanced salt solution was also injected intraperitoneally 30 minutes before or 9 hours after instillation the LPS. In the EP+LPS group, 40mg/Kg of EP was injected 30 minutes before LPS instillation. In the LPS+EP group, 40mg/Kg of EP was injected 9 hours after LPS instillation. The TNF-alpha and IL-6 concentrations in the bronchoalveolar lavage fluid (BALF), and that of NF-KappaB in the lung tissue were measured in the control, LPS and EP+LPS groups at 6 hours after instillation of saline or LPS, and the ALI score and myeloperoxidase (MPO) activity were measured in all four groups 24 and 48 hours after LPS instillation, respectively.RESULTS:
The TNF-alpha and IL-6 concentrations were significantly lower in the EP+LPS group than in the LPS group (p<0.05). The changes in the concentration of these inflammatory cytokines were strongly correlated with that of NF-kappaB (p<0.01). The ALI scores were significantly lower in the EP+LPS and LPS+EP groups compared with the LPS group (p<0.05). In the EP+LPS group, the MPO activity was significantly lower than the LPS group (p=0.019).CONCLUSION:
EP, either administered before or after LPS instillation, has protective effects against the pathogenesis of LPS-induced ALI. EP has potential theurapeutic effects on LPS-induced ALI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bronchoalveolar Lavage Fluid
/
Cytokines
/
NF-kappa B
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Peroxidase
/
Pyruvic Acid
/
Acute Lung Injury
/
Lung
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Tuberculosis and Respiratory Diseases
Year:
2006
Type:
Article
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