Expression of hepatocyte-enriched transcriptional factors during the differentiation of bone marrow mesenchymal stem cell-derived hepatocytes / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 330-334, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-259018
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and distribution of hepatocyte-enriched transcriptional factors during the differentiation of hepatocyte by rat bone marrow stem cells in vitro.</p><p><b>METHODS</b>Mesenchymal stem cells were isolated from rat bone marrow and induced into mature hepatocyte in vitro. The mRNA expression levels of hepatocyte nuclear factor 4 alpha (HNF4 alpha), CCAAT enhancer binding protein (C/EBP) alpha and beta were compared between induced and non-induced cultures using semi-quantitative reverse transcription-polymerase chain reaction. The distribution pattern of HNF4 alpha was detected by immunofluorescence staining and observed by fluorescence microscope.</p><p><b>RESULTS</b>Transcriptional factors HNF4 alpha, C/EBP alpha, and C/EBP beta were expressed in the induced cells during the culture process. The mRNA expression levels of HNF4 alpha and C/EBP alpha were significantly higher in induced cultures than those in non-induced cultures in the early stage, whereas C/EBP beta expression was significantly up-regulated in induced cultures at the late stage (P < 0.05). Immunofluorescence staining showed that HNF4 alpha was located in the cell nucleus of differentiated cells.</p><p><b>CONCLUSION</b>The characteristic time-dependent expression of transcriptional factors HNF4 alpha, C/EBP alpha, and C/EBP beta during the hepatocyte differentiation by bone marrow stem cells demonstrates that the expressions of these transcriptional factors are closely related to the initiation and maintenance of hepatocyte differentiation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Hepatocytes
/
Cell Biology
/
Mesenchymal Stem Cells
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2009
Type:
Article
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