Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 13-16, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-259086
ABSTRACT
<p><b>OBJECTIVE</b>To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.</p><p><b>METHODS</b>Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.</p><p><b>RESULTS</b>In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22.</p><p><b>CONCLUSION</b>SELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Blood Proteins
/
Biomarkers, Tumor
/
Mycosis Fungoides
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Methods
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2009
Type:
Article
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