The mechanism of Stat3 nuclear import / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 299-301, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-259105
ABSTRACT
In order to investigate the mechanism of stat3 nuclear import. positioned a characterized NLS of the SV40 large T antigen into Stat3-GFP, Dstat3-GFP respectively between the C-terminus of Stat3 and the N-terminus of GFP to create Stat3-NLS-GFP and Dstat3-NLS-GFP. With NLS-GFP as the positive control, Expression of the Stat3-NLS-GFP without IL-6 stimulation and Stat3-GFP with IL-6 stimulation resulted in a predominantly nuclear localization in 293T cell. Expression of Stat3-GFP and Dstat3-NLS-GFP without IL-6 stimulation resulted in predominantly cytoplasm localization in 293T cell. The results suggest that latent Stat3 is not anchored in the cytoplasm, and that nuclear localization in response to IL-6 is facilitated by gain of an NLS function.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Cell Nucleus
/
Nuclear Localization Signals
/
Active Transport, Cell Nucleus
/
STAT3 Transcription Factor
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article
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