Intracellular Antibody Fragment Against Hepatitis B Virus X Protein Does Not Inhibit Viral Replication
Yonsei Medical Journal
;
: 721-728, 2006.
Article
in English
| WPRIM
| ID: wpr-25916
ABSTRACT
Replication of the hepatitis B virus is suppressed by deficiency of the X protein. Although several molecules that block cellular targets of X protein reduce the production of hepatitis B virus progeny, the effect of a specific inhibitor of X protein on viral replication has not been investigated. To block X protein specifically, we adopted an intracellular expression approach using H7 single chain variable fragment (H7scFv), an antibody fragment against X protein. We previously demonstrated that cytoplasmic expression of H7scFv inhibits X protein-induced tumorigenicity and transactivation. In this study, intracellular H7scFv expression inhibits reporter gene transactivation but not viral replication determined by endogenous hepatitis B virus polymerase activity assay and real-time PCR. Our findings imply that intracellular expression of antibody fragment against X protein may not be an alternative therapeutic modality for inhibition of hepatitis B virus replication.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virus Replication
/
Immunoglobulin Variable Region
/
Trans-Activators
/
Cell Line
/
Hepatitis B virus
/
Hepatitis B e Antigens
Language:
English
Journal:
Yonsei Medical Journal
Year:
2006
Type:
Article
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