Using single primer PCR to amplify the orf25 gene fragment related to plant male-sterility in Salicornia europaea / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 120-123, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-259182
ABSTRACT
The gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. To clone the orf25 from Salicornia europaea Xinjiang into a T-vector, a single designed primer was used to amplify 1.7kb cDNA fragment with RT-PCR. Sequence analysis reveals that the cloned fragment contains entire orf25 coding region with 98%, 95%, 92% and 88% identity to that of orf25 from Beta vulgaris, Nicotiana, wheat and maize mitochondrion, respectively. This analysis suggests that orf25 gene is highly conserved in terms of evolution in plant; and it also suggests that wild plant Salicornia europaea contain a male-sterility gene similar to crops that is of great importance in improvement of the breed of crop.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Plant Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
Chemistry
/
DNA Primers
/
Chenopodiaceae
/
Reverse Transcriptase Polymerase Chain Reaction
/
Plant Infertility
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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