The preparation of fructose-modified chitosan microcarrier and culture of primary rat hepatocyte / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 116-119, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-259183
ABSTRACT
Crosslinked chitosan microcarries were prepared by the reaction of glutaraldehyde with fructose-modified chitosan. Various factors that influence the preparation were studied and the reaction conditions were optimized. Morphology of rat hepatocyte cultured on chitosan microcarriers was observed using phase contrast microscopy and scanning electron microscope, the metabolic activity was measured. Rat hepatocytes cultured on chitosan microcarrier retained the spherical shape as they have in vivo and had high metabolic activity. Fructose can enhance the metabolic activity of hepatocytes and fructose-modified chitosan microcarrier is a promising scaffold for hepatocytes attachment, which can be used in bioartificial liver support system.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biocompatible Materials
/
Microscopy, Electron, Scanning
/
Cells, Cultured
/
Chemistry
/
Microscopy, Phase-Contrast
/
Cell Culture Techniques
/
Liver, Artificial
/
Hepatocytes
/
Cell Biology
/
Chitosan
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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