Expression of phytase gene in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 112-115, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-259184
ABSTRACT
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aspergillus niger
/
Bombyx
/
Fungal Proteins
/
Blotting, Southern
/
6-Phytase
/
Electrophoresis, Polyacrylamide Gel
/
Genetic Vectors
/
Genetics
/
Hydrogen-Ion Concentration
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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