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2-dimensional gel electrophoresis on protein patterns from suspension cultures of Taxus cuspidate induced by SA / 生物工程学报
Chinese Journal of Biotechnology ; (12): 92-96, 2003.
Article in Chinese | WPRIM | ID: wpr-259188
ABSTRACT
The permeability of cell membrane was enhanced by exogenous SA in the culture of Taxus cuspidata. Nuclei condense and fragments were observed in some cells by using fluorescent microscope, and the degraded chromosomal DNA was observed by using agarose gel electrophoresis. The changes in soluble proteins of the suspension cultures of Taxus cuspidata induced by salicylic acids were analyzed by using two-dimensional polyacrylamide gel electrophoresis. Comparing with the control, seven new protein spots were found and six protein spots were not found in the cultures grown with SA at 48h. The results obtained showed that SA could induce the expression of some special proteins that might be related with the action of SA in the suspension cultures of Taxus cuspidata.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plant Proteins / Electrophoresis, Gel, Two-Dimensional / Salicylates / Gene Expression Regulation, Plant / DNA, Plant / Cell Culture Techniques / Taxus / Electrophoresis, Agar Gel / Genetics Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plant Proteins / Electrophoresis, Gel, Two-Dimensional / Salicylates / Gene Expression Regulation, Plant / DNA, Plant / Cell Culture Techniques / Taxus / Electrophoresis, Agar Gel / Genetics Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2003 Type: Article