Expression of keratin 8 in carbon tetrachloride-induced liver injury of mice / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 37-42, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-259244
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of keratin 8 (K8) in carbon tetrachloride (CCl(4))-induced liver injury of mice.</p><p><b>METHODS</b>Forty ICR mice were divided into four groups. CCl(4) 300 microl/kg body weight in olive oil was injected intraperitoneally for 0, 2, 4, 6 weeks in group A, B, C and D, respectively. Mice were sacrificed 3 d after the last injection and then the vital organs were collected and weighed. RT-PCR and immunohistochemistry methods were used to analyze the expression of keratin 8 in the liver.</p><p><b>RESULTS</b>The ratios of liver and body weight were increased significantly after administration of CCl(4), which were 5.60 %, 6.87%, 7.83 % and 7.76% at 0, 2, 4 and 6 weeks after injection, respectively. The expression of K8 was increased at the 2 w, 4 w and 6 w after CL(4) administration.</p><p><b>CONCLUSION</b>The expression of K8 is positively correlated with the liver injury induced by CCl(4). The accumulation of K8 may be involved in the mechanism of liver injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Carbon Tetrachloride
/
Keratin-8
/
Chemical and Drug Induced Liver Injury
/
Genetics
/
Metabolism
/
Mice, Inbred ICR
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2010
Type:
Article
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