Effect of DRB/alpha-Amanitin on localization of Nrf2 in A549 cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 24-29, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-259246
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of transcriptional inhibitors 5, 6-dichloro-1-b-D-ribofuranosylbenzimidazole (DRB) and alpha-Amanitin on the localization of Nrf2 in the nucleus.</p><p><b>METHODS</b>A549 cells were treated with DRB (50 mg/L) or alpha-Amanitin (2.5 mg/L)for 1 h and 6 h in serum-free medium, respectively. The expressions of Nrf2, HO-1, NQO1 and AKR1C were detected by Western blotting analysis. The localization of Nrf2 was determined by laser scanning confocal microscopy after cells were treated with either DRB or agr-Amanitin for 1 h.</p><p><b>RESULTS</b>The expressions of Nrf2 and Nrf2-ARE gene batteries HO-1, AKR1C and NQO1 were decreased after 6 h treated with either DRB or alpha-Amanitin. The expression of SC35 was up-regulated but RNA Pol II was down-regulated; Y12 and NPC did not significantly change. The localization of Nrf2 in the cell nucleus did not change significantly.</p><p><b>CONCLUSION</b>DRB and alpha-Amanitin can down-regulate the expression of Nrf2 and its targeting proteins HO-1, AKR1C and NQO1, but may have no effect on the localization of Nrf2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Carcinoma, Non-Small-Cell Lung
/
Nucleic Acid Synthesis Inhibitors
/
Cell Line, Tumor
/
Dichlororibofuranosylbenzimidazole
/
Heme Oxygenase-1
/
NF-E2-Related Factor 2
/
Alpha-Amanitin
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS