Proteomics study of intestinal mucosa after ischemic preconditioning against intestinal ischemic reperfusion injury in rats / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 598-602, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-259358
ABSTRACT
<p><b>OBJECTIVE</b>To identify associated proteins involved in the molecular response of ischemic preconditioning (IPC) against intestinal ischemia/reperfusion (II/R) in the intestinal mucosa of rats.</p><p><b>METHODS</b>Sixteen SD rats were randomly divided into II/R and IPC groups. II/R injury in rats was produced by clamping superior mesenteric artery for 60 min followed by 60 min reperfusion. IPC was elicited by 20 min ischemia and 5 min reperfusion before index ischemia. The intestinal mucosa was scratched immediately after 60 min of reperfusion and total proteins were separated by immobilized pH gradient (IPG) based on two-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were analyzed using Image Master 2D Elite 5.0 image analysis software and identified by MALDI-TOF-MS. The biological information of these proteins was searched in the database of these peptide mass finger-printing (PMF). Western blotting and RT-PCR were used to validate the differentially expressed proteins.</p><p><b>RESULTS</b>Image analysis revealed that averages of 1404+/-20 and 1338+/-20 were detected in II/R and IPC groups. A total of 10 spots yielded good spectra, and 8 spots matched with known proteins after database searching. These proteins were mainly involved in anti-oxidation, inhibiting apoptosis and energy metabolism. Western blot confirmed up-regulation of aldehyde dehydrogenase and RT-PCR confirmed up-regulation of aldose reductase in IPC group.</p><p><b>CONCLUSION</b>The clues provided by comparative proteome strategy will shed light on molecular mechanisms of IPC against II/R injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Reperfusion Injury
/
Rats, Sprague-Dawley
/
Ischemic Preconditioning
/
Proteomics
/
Intestinal Diseases
/
Intestinal Mucosa
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2009
Type:
Article
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