Induction of UGT1A1 expression by praeruptorin A and praeruptorin C through hCAR pathway / 药学学报
Acta Pharmaceutica Sinica
;
(12): 794-798, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-259548
ABSTRACT
This study is purposed to investigate the effects of praeruptorin A (PA) and praeruptorin C (PC) on UGT1A1 in HepG2 cells through hCAR pathway. PA and PC were incubated with HepG2 cells for 24 h and 48 h, mRNA and protein expressions of UGT1A1 were determined by real-time PCR and Western blotting assays. Additionally, effects of PA and PC on UGT1A1 mRNA and protein expressions were also measured after transient transfection of a specific CAR siRNA for 72 h in HepG2 cells. UGT1A1 mRNA and protein expression levels were significantly increased by PA and PC after incubation for 48 h. Moreover, the mRNA and protein up-regulations of UGT1A1 were attenuated by transient transfection of a specific CAR siRNA, suggesting the induction was mediated by CAR. The results suggest that PA and PC can significantly up-regulate UGT1A1 expression partially via the CAR-mediated pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Transfection
/
Signal Transduction
/
Chemistry
/
Glucuronosyltransferase
/
Receptors, Cytoplasmic and Nuclear
/
Plant Roots
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2013
Type:
Article
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