Macrophage inflammatory protein-1α promotes the growth of acute myeloid leukemia cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 306-311, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-259594
ABSTRACT
<p><b>UNLABELLED</b>BACKGROWND Macrophage inflammatory protein-1α (MIP-l α/CCL3) belongs to the C-C chemokine family (CCL3), which can be secreted by macrophages, other types of hematopoietic cells and bone marrow stromal cells. Higher levels of MIP-1α were found to be associated with several kinds of hematologic malignancies, including multiple myeloma (MM), chronic lymphocytic leukemia (CLL) and chronic myeloid leukemia (CML). Moreover, MIP-1α has been reported to be an adverse prognostic factor for CLL. However, the impact of MIP-1α on acute myeloid leukemia (AML) has been poorly investigated.</p><p><b>OBJECTIVE</b>To investigate the influence of MIP-1α on proliferction of AML cells.</p><p><b>METHODS</b>Using MLL-AF9 induced AML mouse model, the expression of MIP-1α was measured by real time quantitative RT-PCR. AML cell proliferation was examined by cell counting and colony forming assay (CFC). The influence of blocking the MIP-1α action on the growth and pathogenic ability of AML cells was explored by using the small molecule antagonist for interfering interaction of MIP-1α with its receptor CCR1.</p><p><b>RESULTS</b>The MIP-1α could promote the proliferation and colony formation of AML cells, the blocking MIP-1a could inhibit the growth of AML cells and delay onset of AML.</p><p><b>CONCLUSION</b>The MIP-1a promotes the occurence and progression of AML, therefore blocking the MIP-1α signal pathway may be served as a strategy to inhibit the growth of AML cells, and MIP-1α can be a potential target for treatment of AML.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Leukemia, Myeloid, Acute
/
Macrophage Inflammatory Proteins
/
Cell Line, Tumor
/
Cell Proliferation
/
Chemokine CCL3
/
Chemokine CCL4
/
Receptors, CCR1
/
Multiple Myeloma
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2015
Type:
Article
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