Study on in vitro biomineralization of enamel-binding peptide / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 132-135, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-259682
ABSTRACT
We present the binding ability of a new peptide (CMPQVMPMC-) with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein p III. Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro biomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group (100%). The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Protein Binding
/
Tooth Calcification
/
Chemistry
/
Dental Enamel
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2014
Type:
Article
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