Interaction effect of p53 with HBV in hepatoma cell line SMMC-7721 / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 403-405, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-259970
ABSTRACT
<p><b>OBJECTIVES</b>To study the interaction of hepatitis virus B (HBV) and tumor suppressor p53.</p><p><b>METHODS</b>Plasmid pCMVp53 was transfected or cotransfected with pCMVHBVa (wild type HBV) or PCMVHBVb (mutant type HBV) into the hepatoma cell line SMMC-7721 by lipofectamine. Apoptosis cells were labeled by annexin V-FITC and confirmed by flow cytometry. Reporter plasmids PG13-CAT or p21-luc were cotransfected respectively in each group to indicate transactivation activity of p53 and it's effect on p21 promoter. Western blot was performed to observe p53 expression in each group.</p><p><b>RESULTS</b>The group transfected by pCMVp53 alone exhibit higher luciferase activity and higher apoptosis rate, otherwise, p53 expression, enzyme activity of PG13-CAT or p21- luc and cell apoptosis rate were much higher in the group cotransfected by pCMVp53 and pCMVHBVa, but not in the other cotransfected group; HBV replication was enhanced in p53 cotransfected group.</p><p><b>CONCLUSION</b>p53 expression and effects could be enhanced by HBV and p53 had positive regulation effect on HBV replication.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Virology
/
Virus Replication
/
Transfection
/
Trans-Activators
/
Hepatitis B virus
/
Tumor Suppressor Protein p53
/
Proto-Oncogene Proteins p21(ras)
/
Promoter Regions, Genetic
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article
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