Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 338-340, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-260000
ABSTRACT
<p><b>OBJECTIVES</b>To observe immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen (HBcAg).</p><p><b>METHODS</b>A new DNA vaccine (pSW3891/HBc) encoding for hepatitis B virus core antigen was constructed using plasmid pSW3891 which can be used in human. Control and experiment groups of Balb/c mice were immunized with pSW3891 or pSW3891/HBc by gene gun. Anti-HBc in sera of mice was tested by ELISA (enzyme linked immune sorbent assay). Specific cytotoxicity of cytotoxic T lymphocyte (CTL) of mice was detected by LDH release assay.</p><p><b>RESULTS</b>pSW3891/HBc can express in 293T cell line effectively. Mice immunized with pSW3891/HBc showed strong anti-HBc response and specific high cytotoxicity of CTL.</p><p><b>CONCLUSION</b>pSW3891/HBc induced significantly humoral and cellular immune responses in Balb/c mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
T-Lymphocytes, Cytotoxic
/
Hepatitis B Vaccines
/
Vaccines, DNA
/
Allergy and Immunology
/
Genetics
/
Hepatitis B Antibodies
/
Hepatitis B Core Antigens
/
Immunity, Cellular
/
Mice, Inbred BALB C
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article
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