The role of peroxisome proliferators-activated receptor gamma (PPARgamma) during activation of hepatic stellate cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 216-218, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-260059
ABSTRACT
<p><b>OBJECTIVES</b>To observe the role of PPARgamma during the activation process of hepatic stellate cells (HSC).</p><p><b>METHODS</b>By morphology and RT-PCR, we study the changes of expression of PPARgamma in culture-activated HSC or in vivo activated HSC induced by dimethylnitrosamine (DMN).</p><p><b>RESULTS</b>In vitro, the expression level of PPARgamma in freshly isolated HSC (0.72+/-0.01) significantly reduced to 0.48+/-0.03 on the third day of culture (t = 19.8372, P<0.01), and reduced 70% on the seventh culture-day and could not be detected after the second passage. In vivo, HSC freshly isolated from normal control rats expressed PPARgamma (0.76+/-0.01). During the development of rat liver fibrosis induced by DMN, the expression level significantly reduced to 0.46+/-0.02 after the third injection of DMN (t = 29.5318, P<0.01), and reduced 66% on the end of first week and could not be detected on the end of second and third week.</p><p><b>CONCLUSION</b>The expression of PPARgamma might play an important role on the maintenance of resting-form of HSC, and the reduction of expression of PPARgamma might be an early event during the activation process of HSC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Transcription Factors
/
RNA, Messenger
/
Rats, Wistar
/
Receptors, Cytoplasmic and Nuclear
/
Cell Biology
/
Liver
/
Liver Cirrhosis
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article
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