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Effects of Propofol on The mRNA Expression of Toll-like Receptor 4 in BV-2 Cells During Mimic Ischemia-Reperfusion Injury In Vitro / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 711-713, 2008.
Article in Chinese | WPRIM | ID: wpr-260075
ABSTRACT
This study investigated the effects of propofol on the mRNA expression of Toll-like receptor-4 (TLR4) in BV-2 cells during mimic ischemia-reperfusion (I/R) injury in vitro. BV-2 cells, a mouse microglia line, were cultured and divided into 4 groups at random control group (group C),ischemia/reperfusion group (group I/R), low-dose propofol (25 μmol/L) intervention group (group PF25) and high-dose propofol (100 μmoi/L) intervention group (group PF100). The mRNA expression of TLR4 and NF-kB was measured by means of RT-PCR. TNF-а levels in the supernatants of BV-2 cells were detected by ELISA. The results showed that the mRNA expression of TLR4 and NF-kB was significantly higher in groups I/R, PF25 and PF100 than in group C (P<0.01). And the TNF-а level in the supernatants was elevated in groups I/R, PF25 and PF100 as compared with that in group C (P<0.01). After pre-treatment with propofol, the mRNA expressions of TLR4 and NF-kB and the TNF-ct level were significantly decreased in groups PF25 and PF100 in comparison to those in group I/R (P<0.01). And the decrease in those indicators was more significant in group PF100 than in group PF25 (P<0.01). It was concluded that propofol exerted brain-protecting effects during I/R injury by suppressing the mRNA expressions of TLR4 and NF-kB and deceasing the TNF-а level.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2008 Type: Article