Effect of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 27-31, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-260184
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae (SCAP) in vitro.</p><p><b>METHODS</b>SCAP were isolated and cultured respectively in alpha minimum essential medium (α-MEM) or α-MEM containing 1.8 mmol/L KH2PO4. Alkaline phosphatase (ALP) activity, alizarin red staining, real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the odonto and osteogenic potential of SCAP in the two media.</p><p><b>RESULTS</b>SCAP cultured in α-MEM containing 1.8 mmol/L KH2PO4 exhibited a higher ALP activity [(0.370 ± 0.013) Sigma unit×min(-1)×mg(-1)] at day 3 than control group [(0.285 ± 0.008) Sigma unit×min(-1)×mg(-1)] and KH2PO4-treated SCAP formed more calcified nodules at day 5 [(0.539 ± 0.007) µg/g] and day 7 [(1.617 ± 0.042) µg/g] than those in normal medium [(0.138 ± 0.037) µg/g, P < 0.01]. The expression of odonto- and osteogenic markers were significantly up-regulated after the stimulation of KH2PO4 at day 3 and 7 respectively, as compared with control group.</p><p><b>CONCLUSIONS</b>1.8 mmol/L KH2PO4 can promote the odonto and osteogenic differentiation potential of human SCAP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
Phosphates
/
Phosphoproteins
/
Sialoglycoproteins
/
Stem Cells
/
Osteocalcin
/
Cell Differentiation
/
Cells, Cultured
/
Extracellular Matrix Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2013
Type:
Article
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