Construction of recombinant plasmid with Porphyromonas gingivalis FimA deficiency / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 671-674, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-260212
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant plasmid pPHU281_A_Spec_B, which knock out Porphyrmonas gingivalis (Pg) FimA gene.</p><p><b>METHODS</b>Genomic DNA was extracted from PgATCC33277 which was cultured in anaerobic condition. The upstream and downstream gene of FimA was cloned from Pg genenomic DNA with specific restriction sites by polymerase chain reaction. Suicide vector pPHU281 was inserted by three fragments upstream, downstream of FimA gene and spectinomycin resistance gene. The recombinant plasmid was confirmed by electrophoresis and sequenced after amplification in compentent cells DH-5α.</p><p><b>RESULTS</b>The gene sequence was identified by DNA sequencing analysis. The recombinant plasmid pPHU281_A_Spec_B was successfully constructed.</p><p><b>CONCLUSIONS</b>The recombinant plasmid pPHU281_A_Spec_B was constructed, which may be used for the constructon of FimA deficient Pg.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Recombinant Proteins
/
DNA, Bacterial
/
Base Sequence
/
Sequence Analysis, DNA
/
Porphyromonas gingivalis
/
Fimbriae Proteins
/
Gene Knockout Techniques
/
Genes, Bacterial
/
Genetic Vectors
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2012
Type:
Article
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