Effect of berberine hydrochloride on the secretion of monocyte chemoattractant protein-1 from human periodontal ligament cells in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 610-613, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-260225
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of berberine hydrochloride on the secretion of monocyte chemoattractant protein-1 (MCP-1) from human periodontal ligament cells (PDLC) in vitro culture.</p><p><b>METHODS</b>Periodontal ligament was isolated from extracted human premolars, and PDLC were cultured in vitro. PDLC were divided into two groups, lipopolysaccharide (LPS) group and non-lipopolysaccharide (NLPS) group. Then in accordance with the final concentrations of berberine hydrochloride in cells culture medium (0, 0.01, 0.02, 0.03 g/L), the groups were subdivided into LPS and NLPS control group, LPS1 and NLPS1 group, LPS2 and NLPS2 group, LPS3 and NLPS3 group. Cellular concentration of MCP-1 of each group was determined by enzyme-linked immunosorbent assay (ELISA). The data were statistically analyzed.</p><p><b>RESULTS</b>The MCP-1 contents were not significantly different between the groups of NLPS1, NLPS2 and NLPS3 [(11.33 ± 0.16), (11.45 ± 0.53), (11.25 ± 0.14) ng/L, respectively] and the NLPS control group [(11.32 ± 0.35) ng/L] (P = 0.692, 0.568, 0.524). MCP-1 contents in the groups of LPS1, LPS2 and LPS3 [respectively (38.14 ± 5.34), (34.15 ± 3.36), (26.13 ± 2.12) ng/L] were significantly lower than in LPS control group [(58.42 ± 1.52) ng/L], P = 0.000, 0.000, P = 0.000.</p><p><b>CONCLUSIONS</b>The inhibitory effect of berberine hydrochloride on the activities of MCP-1 from PDLC was more significant when PDLC were stimulated with LPS and in a concentration-dependent manner.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontal Ligament
/
Pharmacology
/
Berberine
/
Bicuspid
/
Cells, Cultured
/
Lipopolysaccharides
/
Chemokine CCL2
/
Bodily Secretions
/
Cell Biology
/
Dose-Response Relationship, Drug
Limits:
Adolescent
/
Child
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2012
Type:
Article
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