Association of microRNA101 expression with clinicopathologic features and prognosis in colorectal cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 365-369, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-260350
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the microRNA101(miR101) expression and its clinical significance in colorectal cancer.</p><p><b>METHODS</b>Tissue microarrays containing 56 specimens of normal mucosa, 51 adenoma and 735 colorectal cancer were examined by locked nucleic acid in-situ hybridization(LNA-ISH) for miR101 expression. Relationship between miR101 expression and clinicopathologic parameters and prognosis of colorectal cancer patients were analyzed. Fresh frozen tissues containing 5 specimens of normal mucosa, 5 adenoma and 47 colorectal cancer were examined by RT-PCR to verify the accuracy of LNA-ISH.</p><p><b>RESULTS</b>Expression of miR101 increased gradually from normal mucosa, adenoma to stage I colorectal cancer (all P<0.01), and decreased gradually from stage II(, stage III( to stage IIII( colorectal cancer (all P<0.01). Overexpression of miR101 was related with lower incidence of lymph node metastasis, lower metastasis rate, higher differentiation and lower recurrence rate (all P<0.01). Multivariate survival analysis demonstrated that miR101 expression was an independent prognostic factor of overall survival (HR=0.550, 95% CI 0.351-0.863) and disease free survival(HR=0.562, 95% CI 0.397-0.794) in colorectal cancer patients. Overexpression of miR101 predicted a better prognosis in colorectal cancer patients.</p><p><b>CONCLUSIONS</b>Expression of miR101 is associated with the genesis and development of colorectal cancer, and may serve as an independent prognostic factor for colorectal cancer patients.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prognosis
/
Colorectal Neoplasms
/
Adenoma
/
Gene Expression Regulation, Neoplastic
/
Multivariate Analysis
/
Disease-Free Survival
/
MicroRNAs
/
Lymphatic Metastasis
/
Neoplasm Staging
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2015
Type:
Article
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