Detection of HBV specific cytotoxic lymphocytes responses in severe hepatitis B patients using MHC-peptide tetramer flow cytometry / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 658-661, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-260640
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role played by HBV antigen specific cytotoxic lymphocytes (CTLs) in severe hepatitis B patients.</p><p><b>METHODS</b>Three kinds of HBV specific CD8+ T cells responding to HBV core 18-27, polymerase 575-583 and envelope 335-343 specific CD8+ T cells were searched with major histocompatibility complex (MHC)-peptide tetramer flow cytometry in the patients we studied. The cytokines produced by these specific CTLs such as IFNgamma, TNFalpha, IL-4, IL-10 were detected using ELISPOT assays. Their ability to lyse target cells was analyzed using a CytoTox96 Non-Radioactive Cytotoxicity Assay kit (Promega).</p><p><b>RESULTS</b>The number of HBV core 18-27 specific CD8+ T cells was significantly higher in the peripheral blood of acute severe hepatitis B group patients than that in chronic severe hepatitis group (P less than 0.05), but lower than that in the acute hepatitis B patients. The IFNgamma and TNFalpha levels of acute severe hepatitis B patients were both significantly higher than those in the chronic severe hepatitis group (P less than 0.05). The ability to lyse target cells of HBV core 18-27 CTLs was also significantly higher in the acute severe hepatitis B group than that in the chronic severe hepatitis B group (P less than 0.05).</p><p><b>CONCLUSION</b>The response to antigen stimulation was much higher in acute severe hepatitis B than in chronic severe hepatitis B patients. The specific CTLs persisted among the peripheral blood mononuclear cells in acute severe hepatitis B, which may be related to the viral clearance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
T-Lymphocytes, Cytotoxic
/
Hepatitis B virus
/
Allergy and Immunology
/
Flow Cytometry
/
Hepatitis B
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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