Determination of magnoflorine in Coptidis Rhizoma and Phellodendri Chinensis Cortex by LC-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3322-3324, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-260661
ABSTRACT
A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis. Samples were extracted by methanol. Agilent Eclipse XDB-C18 ODS column (4.6 mm x 150 mm, 5 microm) was used, and mobile phase was methanol-water (6040) at a flow rate of 0.8 mL x min(-1). Electrospray ionization model (ESI), and MRM model were used for quantification. The linear range of magnoflorine was 4.352-2720 microg x L(-1). The average recovery was above 98%. The method is simple, sensitive and accurate, it can be used for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aporphines
/
Mass Spectrometry
/
Time Factors
/
Drugs, Chinese Herbal
/
Linear Models
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Methods
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
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