Simultaneous determination of four alisols in Rhizoma Alismatis by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3306-3309, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-260665
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of alisol A, alisol F, alisol A 24-acetate and alisol B 23-acetate in the rhizome of Alisma orientalis.</p><p><b>METHOD</b>The separation was performed on a Shim-Pack CLC-ODS a C18 column (6 mm x 150 mm, 5 microm) eluted with the mobile phases of acetonitrile (A)-water (B) in gradient elution. The absorbance was monitored at 210 nm. Orthogonal test was adopted to optimize the extraction conditions of the four alisols.</p><p><b>RESULT</b>The correlation coefficients of the four alisols were higher than 0.999 and the average recoveries were 99.23%, 96.67%, 97.30% and 99.61%, respectively. All the RSDs were less than 3%.</p><p><b>CONCLUSION</b>The validation data demonstrated that the method was accurate and repeatable, and can be used to measure the four alisols in Rhizoma Alismatis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Triterpenes
/
Chemistry
/
Cholestenones
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Rhizome
/
Alisma
/
Chromatography, Reverse-Phase
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS