Research on anti-aging effect of mouse placenta cells transplantation / 生物医学工程学杂志

ABSTRACT

To determine the role of placenta cells allogeneic graft in mammalian longevity, the 15-month-old female BALB/c mice (n = 50) were divided into Control group (A), Short-term transplanted group (B) and Long-term transplanted many times group (C). Their placentae (at 18 days of gestation) were taken out and ground with 50-eye cell grit, and the cells were intraperitoneally injected into the mice of B group and C group three times at intervals of 10 days; then the cells were transplanted into the mice of C group many times till the time of death. The mice were evaluated by use of ultrasound-cardiogram; autopsy; score of cardia, spleen, skin, lung, kidney; histopathology; serum total superoxide dismutase activity, serum maleic dialdehyde content, and serum glutathione peroxidase activity. The long-term surviving stem cells were found to be located in many organ tissues of B and C groups' mice with in situ Y chromosomal hybridization dyeing. Median life span of B group mice was 1.7 times that of A group's after transplantation, but there was no statistically significant difference between B group and C group. Three months after transplantation, in B group, the pathological developments of significant skin, cardia, lung, and kidney were delayed; the retrogradation of heart function was attenuated; the data on heart mass index (mass of heart/mass of body), left ventricular mass and serum Maleic Dialdehyde content, and on spleen mass index (mass of spleen/mass of body), left ventricular diastolic volume, serum Total Superoxide Dismutase activity and serum Glutathione Peroxidase activity, were all in a direction favourable to B group (P < 0.05). These results were in line with the hypothesis, i. e. longevity can be enhanced to some extent by transplantation of placenta cells.