The isolation and assessment of Golgi apparatus from gastric cancer cells SGC7901 / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1085-1088, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-260933
ABSTRACT
The Golgi complex is the central organelle of the secretory pathway and has many complicate functions. The endeavours to isolate and purify the Golgi apparatus from cultured cells will benefit further investigation of Golgi. A large number of gastric cancer cells SGC7901 were cultivated in vitro, then Golgi apparatus were isolated from the cells by differential centrifugation combined with sucrose density gradient ultra-centrifugation. Its purity was characterized biochemically by enzymatic assays, morphologically by electron microscopy (EM) and neutral red supravital staining. Finally the Golgi complex was successfully fractionated from gastric cancer cells SGC7901. The first successful isolation of Golgi apparatus from gastric cancer cells SGC7901 by using ultra-centrifugation will lead to research into the function of Golgi apparatus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Stomach Neoplasms
/
Histological Techniques
/
Cell Line, Tumor
/
Golgi Apparatus
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2010
Type:
Article
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