The cytoskeleton and immigration of laryngeal squamous carcinoma cell affected by Fascin-1 expression / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1034-1038, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-260944
ABSTRACT
This study sought to detect the effect of Fascin-1 expression on the cytoskeleton and immigration of laryngeal squamous carcinoma cell. In the experiment, Fascin-1 expression in Hep-2 cells was inhibited by small interfering RNA. The cytoskeleton of Hep-2 cells was observed with the use of laser scanning confocal fluorescence microscope. Millicell insert was applied to detect the immigration of Hep-2 cells in vitro. The results showed that the integrity of cytoskeleton in Hep-2 cells was broken with the down-regulation of Fascin-1 expression and the immigration ability was decreased significantly (P < 0.05). The inhibiting ratio of cell immigration was 44.6 +/- 6.3%. In conclusion, inhibition of Fascin-1 expression in Hep-2 cells could break the integrity of cytoskeleton and decrease the ability of cellular immigration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cytoskeleton
/
Carcinoma, Squamous Cell
/
Carrier Proteins
/
Down-Regulation
/
Cell Movement
/
Laryngeal Neoplasms
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2010
Type:
Article
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