Effect of shentong zhuyu decoction on pain behavior and spinal cord astrocytes model of osteocarcinoma pain / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the analgesic effect of shentong zhuyu decoction (SZD) and its effect on the expression of the spinal cord glial fibrillary acidic protein (GFAP).</p><p><b>METHODS</b>One hundred C3H/HeNCrlVr male mice were randomly divided into the normal group (n=8), the sham operation group (n=30), the model group (n=30), the Chinese medicine (CM) group 1 (n=8), the CM group 2 (n=8), the CM group 3 (n=8), and the vehicle group (n=8). 0.1 g crude drug of SZD/0.4 mL, 0.3 g crude drug of SZD/0.4 mL, 0.9 g crude drug of SZD/0.4 mL, and 0.4 mL normal saline were respectively given by gastrogavage to mice in CM 1, 2, 3 groups and the vehicle group, once daily for seven days starting from Day 14. The paw withdrawal thermal latency (PWTL), as the behavior indicator, was assessed in mice using radiant thermal stimulator. The lumbar enlargement of the spinal cord was taken after the behavioral test on Day 21. GFAP mRNA and protein expressions were detected using real-time quantitative RT-PCR and Western blot.</p><p><b>RESULTS</b>Compared with the normal group (Day 0) (PWTL 15.91 +/- 1.65 s) and the sham operation group (PWTL Day 4 13.33 +/- 1.44 s; Day 7 11.28 +/- 0.61 s; Day 10 15.47 +/- 2.46 s; Day 14 15.69 +/- 1.98 s; Day 21 15.69 +/- 1.68 s), the PWTL value in the model group (Day 4 13.24 +/- 1.02 s; Day 7 11.30 +/- 1.09 s; Day 10 9.12 +/- 0.54 s; Day 14 7.79 +/- 0.77 s; Day 21 6.36 +/- 0.59 s) progressively decreased (P < 0.05) as time went by, while the spinal cord GFAP mRNA and protein expressions gradually increased. Compared with the normal group (Day 0) and the sham operation group (Day 14), the PWTL value in the CM groups and the vehicle group obviously decreased on Day 14 (P < 0.05). The PWTL value was not significantly different among the model group, CM groups, and the vehicle group on Day 14 (P > 0.05). On Day 21 the PWTL value of CM group 2 and 3 increased and the spinal cord GFAP mRNA and protein expression levels decreased when compared with the model group and the vehicle group (P < 0.05). But no significant difference in the PWTL value or GFAP expression levels was shown among the CM 1 group, the vehicle group, and the model group (P > 0.05).</p><p><b>CONCLUSION</b>SZD had analgesic effect. Inhibition of the proliferation and activation of the spinal cord astrocytes might be one of its mechanisms.</p>