Effect of bushen tiaojing recipe and xiaoyao pill on expression of cathepsin-L mRNA in gonadotropin-primed mice / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 80-84, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-261019
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Bushen Tiaojing Recipe (BTR) and Xiaoyao Pill (XP) on cathepsin-L (Cat-L) mRNA in mice.</p><p><b>METHODS</b>Immature mice were randomly divided into the normal group, the control group, the BTR group and the XP group, three in each group. Cat-L mRNA expression in mice was detected using reverse transcription polymerase chain reaction (RT-PCR) at 0, 4, 8 and 12 h after injecting 5 IU (human chorionic gonadotropin, HCG).</p><p><b>RESULTS</b>Cat-L mRNA expression increased gradually after HCG injection, the relative levels in the control group at 0, 4, 8 and 12 h were 0.066 +/- 0.005, 0.383 +/- 0.045, 0.737 +/- 0.024 and 1.036 +/- 0.073 respectively, comparisons between different time-points showed significant difference (P < 0.01). Compared with the control group, the Cat L mRNA expression was higher at 4 h in both BTR and XP groups (P < 0.01), at 8 h in the XP group (P < 0.05), and at 12 h in BTR group after injecting HCG (P < 0.05). Compared with the control group, cat L mRNA expression showed no statistic difference at 8 h in BTR group and at 12 h in XC group.</p><p><b>CONCLUSIONS</b>BTR promoted the ovulation by enhancing the expression of CatL gene, and that of XP by advancing the peak of CatL gene expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovulation
/
Pharmacology
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Cathepsin L
/
Genetics
/
Gonadotropins
/
Metabolism
/
Mice, Inbred Strains
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2011
Type:
Article
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