Effects of PINK1 gene on cell apoptosis and cell autophagy in neonatal mice with hypoxic-ischemic brain damage / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 263-269, 2016.
Article
in Zh
| WPRIM
| ID: wpr-261247
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of PINK1 (phosphatase and tensin homolog deleted on chromosome ten induced putative kinase 1) gene on cell apoptosis and cell autophagy in neonatal mice with hypoxic-ischemic brain damage (HIBD).</p><p><b>METHODS</b>Seventy-two wild-type C57BL/6 mice and 72 PINK1 gene knockout neonatal C57BL/6 mice were randomly divided into four groups: sham-operated wild-type (SWT), HIBD model wild-type (MWT), sham-operated knockout (SKO) and HIBD model knockout (MKO). HIBD model was prepared by low oxygen exposure for 2.5 hours after right carotid artery ligation. After 24 hours of hypoxia-ischemia treatment, TTC (2,3,5-triphenyl four azole nitrogen chloride) staining was used to measure brain infarct volume. The immunohistochemical staining was used to measure the expression of cell apoptosis protein cleaved-caspase-3 (CC3) in brain tissues. The TUNEL method was used to measure cell apoptosis. The immunofluorescence staining and Western blot were used to measure the expression of cell autophagy protein LC3.</p><p><b>RESULTS</b>Compared with the MWT group, the infarct volume of brain tissues was markedly reduced in the MKO group (P<0.05), the number of apoptotic cells and the cell apoptosis index were markedly decreased in the MKO group (P<0.05), the expression of apoptosis protein CC3 was significantly reduced in the MKO group (P<0.05), the expression of cell autophagy protein LC3 was significantly decreased in the MKO group, and the autophagy indicator LC3II/LC3I was also markedly reduced in the MKO group (P<0.05).</p><p><b>CONCLUSIONS</b>PINK1 gene knockout can protect neonatal mice from HIBD.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Protein Kinases
/
Repressor Proteins
/
Autophagy
/
Apoptosis
/
Hypoxia-Ischemia, Brain
/
Tumor Suppressor Proteins
/
Genetics
/
Animals, Newborn
/
Mice, Inbred C57BL
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2016
Type:
Article