Expression of epidermal growth factor receptor and its relationship to apoptosis in myelodysplastic syndromes / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 22-24, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-261365
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of epidermal growth factor receptor (EGFR) in hematopoietic cells and investigate its relationship to apoptosis in myelodysplastic syndrome (MDS) patients.</p><p><b>METHOD</b>Mononuclear cells from 18 MDS patients were used to analyze the expression of EGFR and the apoptotic signals by immunocytochemical technique and TdT-mediated dUTP nick end labelling (TUNEL) fluorescein. Flow cytometry (FCM) sorted CD(34)(+) cells from 15 MDS cases and 6 normal donors were also analyzed for the EGFR expression and apoptotic signals. Nine normal marrow samples were taken as controls. EGFR expression and its relationship to apoptosis were analysed.</p><p><b>RESULTS</b>(1) There was a higher EGFR expression rate (38.6 +/- 24.6)% in nucleated cells of MDS marrow than in normal marrow (18.1 +/- 14.0)% (P < 0.05). (2) Apoptosis occurred much more in EGFR negative cells (16.1%) than in EGFR positive cells (1.4%) (P < 0.01). EGFR expression showed negative correlation with apoptosis (r = -0.701; t(r) = 3.60; P < 0.01). (3) In CD(34)(+) cells, EGFR expression rate seemed higher in RAEB/RAEB-t/CMML than that in RA/RAS (20.6 +/- 27.9 vs 8.9 +/- 11.8%) subgroup (P > 0.05), while apoptosis was lower in RAEB/RAEB-t/CMML subgroup than in RA/RAS cases (18.2 +/- 12.5 vs 45.2 +/- 20.5%) (P < 0.05).</p><p><b>CONCLUSION</b>There is overexpression of EGFR in MDS cases. And it seems that the overexpressed EGFR represent somewhat the malignant proliferation in MDS and suppress apoptosis through a unknown mechanism.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Myelodysplastic Syndromes
/
Bone Marrow Cells
/
Apoptosis
/
In Situ Nick-End Labeling
/
ErbB Receptors
/
Flow Cytometry
/
Metabolism
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2003
Type:
Article
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