Participation of bonth splenic CD(5)(+) and CD(5)(-) B lymphocytes in production of platelet glycoprotein-specific autoantibodies in chronic ITP / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 460-462, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-261383
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the percentage of splenic CD(5)(+) B lymphocytes in chronic idiopathic thrombocytopenic purpura (IT) and the impact of splenic CD(5)(+) and CD(5)(-) B lymphocytes on the production of platelet glycoprotein (GP)-specific autoantibodies.</p><p><b>METHODS</b>Splenic CD(5)(+) B lymphocytes were identified by two-color flow cytometric analysis in eight patients. Four of the eight patients displayed plasma autoantibodies against both GPIIb/IIIa and GPIb/IX, and their splenic B lymphocytes were separated by Ficoll-Hypaque density gradient and sheep erythrocyte, and further purified by magnetic activate cell separation (MACS). Purified CD(5)(+) and CD(5)(-) B lymphocytes were cultured separately with or without staphylococcus aureus cowan I (SAC). GP specific autoantibodies in culture supernatants were measured by modified monoclonal antibody immobilization of platelet antigen assay (MAIPA).</p><p><b>RESULTS</b>The percentage of splenic CD(5)(+) B lymphocytes in ITP patients was slightly higher than that in control with no statistical significance. MACS purified splenic CD(5)(+) and CD(5)(-) B lymphocytes from three out of four ITP patients produced high levels of anti-GPIIb/IIIa and anti-GPIb/IX antibodies. Culture supernatants of CD(5)(+) B lymphocytes from the other patient showed positive reaction only in GPIb/IX MAIPA. Culture supernatant of CD(5)(-)B lymphocytes from the same patient were double positive in both GPIIb/IIIa and GPIb/IX MAIPA.</p><p><b>CONCLUSIONS</b>Both splenic CD(5)(+) and CD(5)(-) B lymphocytes produce platelet GP-specific autoantibodies in chronic ITP with similar antibody spectrum and titer, and may all play a role in the autoimmune pathogenesis of ITP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Autoantibodies
/
Spleen
/
B-Lymphocytes
/
Chronic Disease
/
Purpura, Thrombocytopenic, Idiopathic
/
Platelet Glycoprotein GPIIb-IIIa Complex
/
Platelet Glycoprotein GPIb-IX Complex
/
CD5 Antigens
/
Cell Biology
/
Allergy and Immunology
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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