Effects of novel human chemokine-like factor 1 (CKLF1) on bone marrow hematopoietic stem cell/progenitor cell in vitro / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 301-303, 2002.
Article
in English
| WPRIM
| ID: wpr-261406
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of a novel human chemokine-like factor 1 (CKLF1) on stem cell/progenitor cells.</p><p><b>METHODS</b>Human bone marrow mononuclear cells were separated by Ficoll (1.077 g/ml), and cultured in suspension and semisolid colony culture. The effects of CKLF1 on CFU-GM and CFU-Mix were observed.</p><p><b>RESULTS</b>The number of CFU-GM was significantly increased in 10 groups by addition of CKLF1 plasmid supernatant. The mean value was 234.81 +/- 98.71/1 x 10(5) cells, 2.42 fold of control group (P < 0.05). The mean value of CFU-Mix in groups of negative control, CKLF1, PHA, GM-CSF and G-CSF were 82.00 +/- 20.25, 105.76 +/- 36.70, 146.63 +/- 27.09, 143.33 +/- 60.23 per 1 x 10(5) cells, respectively, no statistical differences were seen between control and CKLF1 groups. The CD(34)(+) cells were detected by FACS. The average percentage in the groups of normal control, CKLF1, PHA and GM-CSF were (0.75 +/- 0.62)%, (1.32 +/- 0.87)%, (0.15 +/- 0.02)%, and (0.29 +/- 0.23)%, respectively. Compared with control, no significant differences were seen between each group (P > 0.05).</p><p><b>CONCLUSION</b>Novel chemokine-like factor 1 can increase the proliferation of CFU-GM, which indicated that CKLF1 could increase the proliferation of human bone marrow hematopoietic progenitor cells and colony formation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Hematopoietic Stem Cells
/
Cells, Cultured
/
Chemokines
/
Cell Biology
/
MARVEL Domain-Containing Proteins
Limits:
Adult
/
Humans
Language:
English
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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