WT1 downregulation during K562 cell differentiation and apoptosis induced by bufalin / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 356-359, 2002.
Article
in English
| WPRIM
| ID: wpr-261414
ABSTRACT
<p><b>OBJECTIVE</b>To study the change of WT1 gene expression during human leukemic K562 cell differentiation and apoptosis induced by bufalin.</p><p><b>METHODS</b>Cell viability was determined by trypan blue exclusion, cell differentiation and apoptosis by nitro blue tetrazolium (NBT) reduction test, morphology and flow cytometry, expression of WT1 protein by Western blot analysis, and expression of WT1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>(1) The cell proliferation was inhibited by bufalin and the IC(50) at 24, 48, 72 h were 0.026, 0.032 and 0.006 micro mol/L, respectively. (2) Bufalin induced K562 cell differentiation towards macrophage/monocyte within concentration from 0.01 to 0.05 micro mol/L and apoptosis at higher than 0.026 micro mol/L. (3) The expression of WT1 protein and mRNA were downregulated by bufalin in the initial stage of differentiation and apoptosis induced by bufalin.</p><p><b>CONCLUSION</b>K562 cell differentiation and apoptosis induced by bufalin might relate to the downregulation of WT1 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Down-Regulation
/
Cell Differentiation
/
Apoptosis
/
WT1 Proteins
/
Cell Proliferation
/
Genetics
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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