Expression of B7 costimulator in eight human malignant hematological cell lines and its significance / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 345-348, 2002.
Article
in English
| WPRIM
| ID: wpr-261417
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.</p><p><b>METHODS</b>Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.</p><p><b>RESULT</b>Eight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.</p><p><b>CONCLUSION</b>Multiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
T-Lymphocytes
/
Transfection
/
Cell Line
/
Interleukin-2
/
B7-1 Antigen
/
U937 Cells
/
Allergy and Immunology
/
Flow Cytometry
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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