Study on the in vivo killing activity of YCD/5-FC gene therapy system on K562B cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 173-175, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-261435
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the killing activity of yeast cytosine deaminase/5-fluorocytosine (YCD/5-FC) gene therapy system on gene-transferred tumorigenic cell line K562B in vivo.</p><p><b>METHOD</b>K562B cell was infected with high titer virus and a gene transferred cell clone, YCD-K562B, was selected. Twelve male SCID mice of 4 week old were divided into 2 groups at random and both YCD-K562B and K562B cells were implanted to each mice. 5-FC or saline was given i. p for 10 days after tumor developed, and relative tumor volume was measured every 3 days. At the end of experiment, animals were sacrificed and the specimens were processed for histopathological examination.</p><p><b>RESULTS</b>At the end of experiment (21 days after tumor cell implantation), the relative tumor volume of the 4 groups were YCD-K562B + 5-FC 2.922 +/- 0.581, YCD-K562B + saline 24.434 +/- 4.790, K562B + 5-FC 22.701 +/- 2.350 and K562B + saline 24.460 +/- 1.670; t-test analysis showed that 5-FC could kill cells (YCD-K562B) in vivo (P = 0.0001), but had no effect on the growth of gene-untransferred cells (K562B) (P = 0.096). In YCD-K562B + 5-FC group, relative tumor volume reduced in 3 approximately 6 days after treatment (the minimum was 0.681). Necrosis around artery could be found in the tumor of YCD-K562B + 5-FC group.</p><p><b>CONCLUSION</b>YCD/5-FC suicide gene therapy system has a significant in vivo killing activity to gene-transferred tumorigenic YCD-K562B cell.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Saccharomyces cerevisiae
/
Therapeutics
/
Transfection
/
Genetic Therapy
/
Treatment Outcome
/
Mice, SCID
/
K562 Cells
/
Xenograft Model Antitumor Assays
/
Cytosine Deaminase
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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