Diamide and cyclosporin A enhanced arsenic trioxide-induced apoptosis in NB4 cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 254-257, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-261437
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of mitochondrial membrane permeability transition pore (MPT)-opened agent diamide and MPT-closed agent cyclosporin A on arsenic trioxide (As(2)O(3))-induced apoptosis in acute promyelocytic leukemia (APL) cell line NB4.</p><p><b>METHODS</b>NB4 cells were treated with As(2)O(3) alone or in combination with diamide or cyclosporin A in different concentrations. Cell apoptosis was assessed by the morphological observation, Annexin-V assay, distribution of cellular DNA contents and genomic DNA electrophoresis. The mitochondrial transmembrane potentials (DeltaPsim) were detected by flow cytometry according to the intensity of rhodamine 123 uptake in cells.</p><p><b>RESULTS</b>Both diamide and cyclosporin A significantly enhanced As(2)O(3)-induced apoptosis in NB4 cells. The DeltaPsim collapse induced by As(2)O(3) was also enforced by combined treatment with diamide or cyclospo-rin A. 1 micromol/L As(2)O(3) alone treatment for 72 hours led to DeltaPsim disruption in 27.9% of cells, while combined treatment of As(2)O(3) and diamide or cyclosporin A increased DeltaPsim disruption cells to 59.7% and 42.2%, respectively.</p><p><b>CONCLUSIONS</b>As(2)O(3)-induced DeltaPsim disruption possibly involves with thiol oxidation or crosslink of important components especially ANT-related molecules.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxides
/
Pathology
/
Pharmacology
/
Physiology
/
Arsenicals
/
Sulfhydryl Reagents
/
Tumor Cells, Cultured
/
Leukemia, Promyelocytic, Acute
/
Cyclosporine
/
Apoptosis
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS