Detection of inv (16) in acute myelomonocytic leukemia by interphase fluorescence in situ hybridization / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 30-32, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-261445
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate fluorescence in situ hybridization (FISH) in the detection of inv (16) (p13; q22).</p><p><b>METHODS</b>Spectrum red labeled yeast artificial chromosome (YAC) clone 854E2 which spans the breakpoint cluster region in MYH11 in band 16p13 and single color interphase FISH were used to detect inv (16) in 26 cases of acute myelomonocytic leukemias (AML-M(4)), and the results were compared with that of conventional cytogenetic analysis.</p><p><b>RESULTS</b>R banding karyotyping test revealed no inv (16) in 25 cases, one AML M(4Eo) case showed inv (16) by G banding. Nine cases including all three M(4Eo) had inv (16) by FISH analysis, among whom the characteristic fluorescence signal pattern of the inv (16) was seen in 13.3% to 32.1% (median, 21.3%) of the tested cells.</p><p><b>CONCLUSION</b>YAC 854E2 and interphase FISH provide a powerful technique in the detection of inv (16) (p13q22).</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Chromosomes, Human, Pair 16
/
Leukemia, Myelomonocytic, Acute
/
In Situ Hybridization, Fluorescence
/
Genetics
/
Interphase
/
Chromosome Inversion
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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